ACT has developed proprietary protocols for the differentiation of Cord Blood Stem Cells into specialized stem cell lines. Specific progenitor cells provide enhanced homing, differentiation and engraftment potential in their target areas. The following specialized lines will be available through ACT during 2006:

CELL TYPE	AREA OF REGENERATION	APPLICABLE CONDITIONS
1. Neuronal	brain, spinal cord	MS, ALS, PD, SCI, CP
2. Dendritic Cells	immune system	HIV, Hep C, Cancer
3. Retinal	eyes	RP, MD, Glaucoma
4. Hepatocytes	liver	Hep B/C, liver disease
5. Pancreatic Islet	pancreas	diabetes
6. Epithelial	skin, scalp	skin, anti aging, hair
7. Endothelial organ	membrane, vessel, skin	heart disease, skin
8. Osteoblasts	bone	bone injury
9. Chondrocytes	cartilage	arthritis, joint injury
10. Muscle	muscle	muscular dystrophy

Specialized neuronal cells are also being generated in the areas of:

1) Dopaminurgic Neurons	(L-Dopa producing neurons)	Parkinson's Disease
2) Motor Neurons	(anterior horn cells)	ALS
3) Glial Cells	(myelin producing cells)	Multiple Sclerosis

No matter how powerful a cell is, its viability and clonogenic capacity are always compromised to some degree by the freezing process. The commonly used liquid freeze media with small amounts of the natural cryoprotectant DMSO are effective, however a significant amount of viability is lost (cells die on defrost or become ineffective) using traditional methods. This is due to the formation of ice crystals and osmotic stress on the cell wall created by the density changes in liquid as it freezes.

An optimized freeze media has been developed which ensures greater viability and clonogenic capacity on defrost, approaching the performance of a cell which has never been frozen at all. A similar media has been developed which "holds" or localizes the cells in a particular areas to enhance engraftment potentials. This is particularly applicably to regeneration of skin, joints and other topically accessible conditions.